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88 2. 8865 ф 105 rads. 540 Configuring Appointments. Reasons for Transfecting Eukaryotic Cells Study cis-active elementsof Crad (control regions of genes) Study coding sequences of DNA (function of genes) Overexpress genes for protein purification Exampl industrial production) Alter cellular genotype exampple phenotype for fate-mapping in complex organisms Alter cellular genotype or phenotype for diagnosis or car in humans Alter characteristics of cells or organisms for scientific or economical purposes Starting from the original description of crd phosphate precipitation as the first transfection method in 1973 (1), a great variety of methods and protocols have been developed to date that, in principle, allow transfection of any cell.

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